Eight kinds of white-rot fungi were used to decolorize two different dyes, Congo red (azo dye) and crystal violet (tritane dye). The fungi were co-cultured with the dyes for five days. Result indicated that the decolorization rate of the Congo red reached as high as 99 percent by Tyromyces albidus and Irpex lacteus, while that of the crystal violet reached 90.62 percent by Pycnoporus sanguineus. The lignin oxidative systems of the eight fungi were examined in quality. Under the same culture conditions, laccase activity had a significant influence on dye decolorization. Fungi cultured in shaking conditions showed a higher dye decolorization percentage when compared with those cultured in static conditions. Three kinds of white-rot fungi selected in the former experiment were applied in the further study to decolorize mixed dyes. Result showed that these white-rot fungi could decolorize the mixed dyes as well as the sole dye. Meanwhile, the decolorization by the single strain was better than that by the mixed stains.