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白腐菌对简单混合染料的脱色

Decolorization of Simple Mixed Dyes by White-rot Fungi

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选取8株白腐菌分别与偶氮类染料刚果红和三苯甲烷类染料结晶紫共培养5d,进行脱色菌种筛选,发现白干酪菌和白耙齿菌对刚果红的脱色率均达到99%,血红密孔菌对结晶紫的脱色率达到90.62%.结合木素氧化酶系定性检测的结果,证明在相同培养条件下,漆酶活性是影响染料脱色率的主要因素;在振荡培养方式下菌种对染料的脱色率高于静置培养;利用筛选出的高效脱 色菌对简单混合染料进行脱色试验,同样达到了很好的效果,表明单一菌种的脱色效果要好于混合菌种.
Eight kinds of white-rot fungi were used to decolorize two different dyes, Congo red (azo dye) and crystal violet (tritane dye). The fungi were co-cultured with the dyes for five days. Result indicated that the decolorization rate of the Congo red reached as high as 99 percent by Tyromyces albidus and Irpex lacteus, while that of the crystal violet reached 90.62 percent by Pycnoporus sanguineus. The lignin oxidative systems of the eight fungi were examined in quality. Under the same culture conditions, laccase activity had a significant influence on dye decolorization. Fungi cultured in shaking conditions showed a higher dye decolorization percentage when compared with those cultured in static conditions. Three kinds of white-rot fungi selected in the former experiment were applied in the further study to decolorize mixed dyes. Result showed that these white-rot fungi could decolorize the mixed dyes as well as the sole dye. Meanwhile, the decolorization by the single strain was better than that by the mixed stains.

White-rot fungiMixed dyesLaccaseDecolorization

李超、魏兴东、汪春蕾、赵敏

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东北林业大学,哈尔滨,150040

白腐菌 混合染料 漆酶 脱色

世界自然科学基金世界自然科学基金国家自然科学基金国家自然科学基金

WWFCN0078.013017077530671702

2009

东北林业大学学报
东北林业大学

东北林业大学学报

CSTPCDCSCD北大核心
影响因子:0.74
ISSN:1000-5382
年,卷(期):2009.37(6)
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