Establishment of Suspension Culture System of Protocorm-like Bodies for Dendrobium candidum
An experiment was conducted to study the effects of different concentrations of MS culture medium, NAA, sucrose, coconut milk, and inoculation density on proliferation of protocorm-like bodys (PLBs) of Dendrobium officinale in suspension culture. Results showed that 1/2MS medium supplemented with 1.0mg/L NAA, 50g/L coconut milk, and 3 g/L sucrose was optimal for the proliferation of the PLBs. Inoculation with 40 g PLBs in 1 liter of liquid medium was most suitable for PLBs proliferation.
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NSTL
万方数据
维普
