Tissue Culture and Rapid Propagation of Melastoma intermedium
An experiment was conducted to study the tissue culture and rapid propagation of Melastoma intermedium Dunn, an epidemic species of wild flowers in China. Results showed that the axillary buds of cultured stem segments of M. intermedium were germinated on MS medium. The induction rate on MS medium supplemented with 6-BA 1.0 mg·L-1 and NAA 0. 5 mg·L-1 was 80%. Eight-three percent of clustered buds were formed at the base of inoculated buds after subculture on MS containing 6-BA 0.5 mg·L-1 and NAA 0.05 mg·L-1 or MS containing 6-BA 0.5 mg·L-1 and NAA 0. 1 mg·L-1. The proliferation coefficient per month was 3. 6. The rooting rate reached 100% after rooting culture for one month. The optimal medium for rooting was 1/2MS supplemented with NAA 0.1 mg·L-1. The rooted seedlings were transplanted into the culture substrate containing peat, perlite and humus soil at a volume ratio of 1 : 1 : 1, and the average survival rate was up to 87%.
NETL
NSTL
万方数据
维普
