切花菊悬浮细胞系的建立及生长规律
Establishment of Cell Suspension Culture and Growth Regularity of Dendrathema morifolium
时颂 1李青1
作者信息
- 1. 国家花卉工程技术研究中心(北京林业大学),北京,100083
- 折叠
摘要
以切花菊‘粉贵人’(Dendrathema morifolium‘ Fenguiren’)淡黄色、质地疏松、增殖速度快的愈伤组织为起始材料,接种在MS+2,4-D0.5 mg/L+6-BA0.2 mg/L+30 g/L蔗糖的液体培养基上,在23℃黑暗条件下振荡(100 r/min)培养30 ～40 d,建立分散性好、均匀、生长迅速的悬浮细胞系.在悬浮细胞系培养过程中研究了悬浮细胞的生长规律,结果表明:在40 mL培养液中,接种细胞起始数量最适为1.6×103 ～4.8×103个；pH值范围在5.29～5.68时,悬浮细胞增殖率最高,继代时保留1/3体积的旧培养液有利于其细胞增殖.
Abstract
Dendrathema morifolium ' Fenguiren' of pale yellow callus with loose structure and high proliferation rate was selected as material for cell suspension culture.It was cultured in modified liquid MS medium MS+2,4-D0.5 mg/L+6-BA0.2 mg/L+30 g/L, and shook at 100 r/min under dark condition at 23 ℃.About 30-40 days later, stably growing cell suspension cultures were established.The growth pattern of the cultures was investigated.The results showed there are 1.6× 103-4.8×103 initial inoculation cells in the liquid medium of 40 mL.In 5.29-5.68 of pH, suspension cell has the highest rate of cell proliferation.1/3 of old liquid medium left is fit for cell proliferation during subculture.
关键词
切花菊/悬浮细胞培养/细胞增值率Key words
Dendrathema morifolium/Cell suspension culture/Rate of cell proliferation引用本文复制引用
基金项目
国家林业局林业公益性行业科研专项项目(200904050)
出版年
2013
国家科技期刊平台
NSTL
维普
万方数据