Pathogen Identification of Jacaranda acu tifolia Stem Rot and Its Phylogente ic Analysis
By observing the fixed sample plots , we discovered a new fungal disease named Jca arandaa cutifolia stem rot, mainly damaging to the stem of Jacaranda acutifolia Humb .Et Bonpl , on J.acutfi olia planting regions in Xichang , Sichuan Prov-ince, and recorded the symptoms .We collected the junctions of diseased and healthy tissues , and determined isolated , cultured with single spore , and the pathogenicity .We obtained A1 and B1, as two separate dominant strains , by morpho-logical and developmental systems rDNA-ITS sequences .Two strains in morphological characteristics were consistent with Fusarium oxysporum and Fusarium chlam ydosporum,respectively .Using the fungal universal primers ITS 1 and ITS4, the internal transcribed spacer rDNA sequences of them were amplified , then after recyclying , cloning and sequencing the am-plification products , two longth of 516 and 523bp gene fragments were got severally .By the results of Blast in Genbank , the analysis of fungal rDNA systemic development and phylogenetic tree , we confirmed the pathogens of J.acutifoliastem rot and these two fungal pathogen strains A 1 and B1 were identified as F.oxysporum and F.chlamydosporum, respectively.
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