Relationsh ip betwe en th e Induction of Embryogenic Cla lus of Larch and the Mo rphology of Immature Embryos
With immature embryos for embryogenic callus induction , we induced embryogenic callus from zygotic embryos of Lar-ix olgensis 73-44, L.kaempfer 5*, L.kaempferi 5, L.kaempferi 5×L.olgensis 77-1, L.kaempferi 5×L.gmelinii 9 and L. kaempferi 12×L.gmelinii 9.The embryogenic callus induction of immature embryos of L.kaempferi 5×L.olgens si 77-1 was the highest in July 1 st, which was 13.93%.There was a certain difference in the morphology of the different acquisition time of the larch , and the callus formation was different and the callus induction was significantly influenced by the time and the family.Polyembryonic phenomenon was in the development of larch embryos .The embryogenic callus induction rate was negatively correlated with loose powder after days and embryo length , and was a significant positively correlated with polyembryonic occurrence rate ( P=0.028) .