Rapid Propagation System of Catalpa bungei and C.fargesii Bur.f.duclouxii
To promoting seedling production , we selected superior individuals of C.bungei ( QS2) and C.fargesii Bur.f.du-clouxii ( DQ72) to establish its effective rapid propagation system .Proper sterilization method of stem segments was treated by 0.1%HgCl2 for 6.5 min.16.67%and 19.79%of browning rate, as well as 18.75%and 21.88%of contamination rate were observed in QS 2 and DQ72 after inoculated for 20 d, respectively .DKW ( Driver and Kuniyuki Walnut ) basic medi-um was suitable for tissue culture of QS 2 and DQ72.The optimum initial culture medium was DKW+0.8%agar+1 g· L-1 activated carbon+2.5%sucrose+1.0 mg· L-1 6-BA+0.1 mg· L-1 IBA indicated by 84.38%and 87.50%of germination rate of axillary buds, respectively, in QS2 and DQ72 after growing for 30 d.The best medium for cluster buds induction was DKW+0.6%agar+2.5%sucrose+6-BA 3.0 mg· L-1+0.2 mg· L-1 IBA.Q2S and DQ72 proliferated strong shoots and displayed multiplication factor of 6.88 and 6.63 after 35-day growth, respectively.The best rooting medium was DKW +0.8%agar+2.5%sucrose+0.2 mg· L-1 NAA+0 .1 mg· L-1 IBA with 100%of rooting rate in both of QS 2 and DQ72 after growing for 30 d.In the medium, QS2 displayed root number of12 .1and root length of 5.3 cm in average.While, in DQ72 these were 18.5 and 3.2 cm, respectively.The survival rate of QS2 and DQ72 were 92.3%and 96.6%, respectively, after trans-planted for 60 d.
NETL
NSTL
万方数据
维普
