Forestry University,2017,45(7):66-68.With the flowers and trees Hydrangea buds as explants, we studied the effects of different disinfection time for explants of different types of pollution effects on bud germination rate, different culture medium for proliferation and rooting of Hydrangea large flowers.The disinfection method suitable for large flowers and trees Hydrangea was 0.1% HgCl2 for 8-min disinfection.With a higher lateral the bud germination rate can reach 72.5%.The proliferation medium was WPM+IBA1.0mg/L+NAA0.75mg/L.The most suitable rooting medium was 1/4WPM+NAA1.0mg/L.