Comparison on Fecundity and Migration Ability of Bursaph elenchus xylophilus with Different Virulence
The virulence of different strains of Bursaphelenchus xylophilus was determined by challenging with Pinus thunbergii and P.massoniana.The strains with different virulence were screened and their biological characteristics were compared. Strains AMA3C28 and USA were more virulent than C14-5 and OKD-1.The wilting ratio on P.thunbergii was 100%for 35 d after inoculation with AMA3C28 and USA, while on P.massoniana were 75%and 20%, respectively.C14-5 and OKD-1 had no or very weak pathogenicity on both P.thunbre gii and P.massoniana.AMA3C28 and USA proliferated rap-idly in P.thunbergii seedlings 35 d after inoculation, which was significantly higher than that of C14-5 and OKD-1.The fecundity of AMA3C28 and USA was significantly higher than that of OKD-1 and C14-5 when feeding on B.cinerea.The sex ratio of male and female in AMA3C28 and USA was significantly higher than that in C14-5 and OKD-1.Difference was also observed in tail morphology of adult female between virulent and avirulent strains when feeding on Botrytis cinerea. For strong virulent strains ( AMA3C28 and USA) , 100%adult females had no terminal mucro.However, about 28%of adult females of OKD-1 had terminal mucro and 2%of adult females of C14-5 had terminal mucro.The migration ability of AMA3C28 and USA in the stem segment of P.thunbergii was higher than that of C14-5 and OKD-1.