The Effect of Brassinolide Induction on the Mass Fraction of Secondary Metabolites and Aphid Resistance in Lyci-um barbarum Leaves
To explore the induction of insect resistance in Lycium barbarum by brassinolide(BR),aiming to reduce the use of chemical pesticides and lower environmental pollution,an experiment was conducted with potted L.barbarum seedlings.The leaves were sprayed with a BR solution,and the yields of secondary metabolites in the leaves were measured at differ-ent induction concentrations and times.This included the mass fractions of tannins,mass concentrations of betaine,total phenolics,and total flavonoids,while also observing the growth and development of Aphis gossypii Glover.The results showed that BR induction significantly influenced the yield of secondary metabolites in L.barbarum leaves.After 21 days of induction with l.00 mg/L of BR,the mass fraction of tannins,along with the mass concentrations of betaine and total phe-nolics,were 4.78,1.24,and 1.55 times higher than the control(CK),respectively.Furthermore,after 21 days of induc-tion with 0.10 mg/L of BR,the total flavonoid mass concentration was 1.59 times that of the CK.Additionally,the BR-treated L.barbarum leaves resulted in a decreased survival rate of A.gossypii Glover,extended total nymphal duration,and reduced total offspring production.In summary,BR induction primarily enhanced the yield of secondary metabolites in L.barbarum leaves and exhibited inhibitory effects on the growth,development,and reproduction of A.gossypii Glover.