Metabolomic Commonality in the Response of Five Pine Tree Species to Pine Wood Nematode Stress
In order to investigate the molecular defense mechanisms of pine trees under pine wood nematode stress,five repre-sentative pine tree species,namely Pinus tabuliformis Carrière from Fushun,Liaoning(LY),Pinus koraiensis from Fush-un,Liaoning(LH),Pinus densiflora from Weihai,Shandong(SC),Pinus thunbergii from Weihai,Shandong(SH),and Pinus massoniana from Changsha,Hunan(HM),aged between 15-20 years,were selected as study subjects.Non-targe-ted metabolomics using liquid chromatography-mass spectrometry(LC-MS)technology was employed to categorize the aforementioned five pine tree species into healthy and diseased groups.The differential metabolite distribution,common characteristics of differential metabolites,significance of common differential metabolite expressions,distribution of en-riched metabolic pathways,and common characteristics of differential metabolic pathways among the five groups of pine tree samples were investigated.The results showed that the metabolic products of the five pine tree species infected with pine wood nematodes were concentrated in seven compound classes,including benzene and substituted derivatives,carbox-ylic acids and their derivatives,fatty acyls,phenols,prenol lipids,steroids and their derivatives,and other classes.A-mong the combinations of HM,LH,LY,and SC,as well as HM,LY,SC,and SH,only one common significantly differ-ential metabolite was detected,which were thymol and 6-paradol.There were no common significantly differential metabo-lites shared among the five pine tree species or the remaining four pine tree combinations.After being infected with pine wood nematodes,the expression level of 6-paradol significantly increased in HM,LY,SC,and SH,while there was no significant difference in LH.The expression level of thymol significantly increased in HM,SC,LY,and LH,with no sig-nificant difference in SC.Each of HM,LH,LY,SC,and SH enriched in 51,55,46,71,and 43 significantly differential signaling pathways,respectively.There were 19 common significantly differential signaling pathways among the five pine tree species,mainly related to plant stress responses,including pathways such as arachidonic acid metabolism,linoleic acid metabolism,phenylpropanoid biosynthesis,and various amino acid metabolism.
万方数据
维普
