Abstract
Amaranthus retroflexus L.is a serious and widespread malignant weed in soybean fields in Heilongjiang Province.Exploring the dormancy characteristics of A.retroflexus L.seeds and the physiological response of its seedlings to acifluorfen sodium can provide a basis for further researches on its resistance mechanism.Using newly harvested and stored A.retroflexus L.seeds for one year as experimental materials,the effects of different concentrations of HCl,NaOH,water temperature,gibberellic acid(GA)and polyethylene glycol(PEG)on the dormancy and germination of A.retroflexus L.seeds were studied.The sensitivity of A.retroflexus L.to acifluorfen sodium was determined using bioassay.The effects on leaf chlorophyll content and target enzyme activity were studied at a normal dosage of 360 g a.i.hm-2 and a doubling dosage of 720 g a.i.hm-2 of acifluorfen sodium.Newly harvested seeds exhibiting dormancy were soaked in water of various temperatures and in different concentrations of NaOH and HCl,which were ineffective in breaking the seed dormancy.GA could break seed dormancy,and the highest seed germination rate reached 93.33%when they were soaked at 3 000 mg·L-1 for 72 h and 4 000 mg·L-1 for 48 h.The drought stress was simulated with a 15%-25%polyethylene glycol solution,which had no significant effect on the seed germination rate.The GR50 value of acifluorfen sodium for A.retroflexus L.was 705.7 g a.i.hm-2,which was 1.96 times the recommended dose in the field.After the application of different doses of acifluorfen sodium,the chlorophyll content of A.retroflexus L.reached its minimum value 3 days after treatment(DAT),and then gradually increased.The activity of the target enzyme protoporphyrinogen oxidase(PPO)reached the highest value at 7 DAT under different dosages,and gradually returned to normal levels thereafter.Soaking with gibberellin was an effective method to break seed dormancy.A.retroflexus L.seeds had certain drought resistance during the germination process.A.retroflexus L.was not sensitive to acifluorfen sodium and acifluorfen sodium ether,and could not effectively inhibit the PPO activity,indicating that A.retroflexus L.had target resistance to acifluorfen sodium.
基金项目
National Major Special Project for the Cultivation of New Genetically Modified Biological Varieties(Topic ZX08011-003)
NETL
NSTL
万方数据