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Cloning and Bioinformatics Analysis of CsFK111 Gene from Cucumbers

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Cloning and Bioinformatics Analysis of CsFK111 Gene from Cucumbers
At the early stage,the transcriptome sequencing technique was used to detect the differentially expressed gene CsFK111 between vine cucumber and dwarf cucumber D0462.The gene was cloned,and bioinformatics software tools were used to analyze and predict the gene family and this gene.There were 30 members of the cucumber F-box gene family.The coding region of the cucumber CsFK111 gene was full-length 1 314 bp,which encoded 437 amino acids and was predicted to be located in the nucleus.The protein encoded by this gene was a non-transmembrane protein,and the prediction of the secondary structure showed that β-lamellar structure and irregular crimp were dominant.A comparison of the phylogenetic tree showed that it was closest to cantaloupe and belonged to the same branch.The results provided a basis for future study on the regulation mechanism of the CsFK111 gene on cucumber dwarfing and also laid a foundation for further study of FBK family proteins.

F-box genedwarf cucumbergene cloningbioinformatics analysis

Zhang Hetong、Li Li、Gao Mei、Jia Jincui、Xin Ming

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College of Horticulture and Landscape Architecture,Northeast Agricultural University,Harbin 150010,China

F-box gene dwarf cucumber gene cloning bioinformatics analysis

2024

东北农业大学学报(英文版)
东北农业大学

东北农业大学学报(英文版)

影响因子:0.075
ISSN:1006-8104
年,卷(期):2024.31(4)