Analysis of Polysaccharides Content in Orange Skin
Polysaccharides were extracted from orange skin by alcohol precipitating-water extracting method. Three factors influencing extraction performances, such as extraction temperature, extraction time and ratio of material to water were investigated through orthogonal experiment design. The optimum extraction conditions were determined : the ratio of material to water 1:35, extraction temperature 90℃, and the extraction time 120 min. Phe-nol-sulfuric acid method was employed to determine polysaccharides content in orange skin. The results show that the conversion factor of polysaccharides in orange skin is 3.15, the average mass fraction is 2.36%, and the relative standard deviation (RSD) is 2.14%.