In oilfield water injection development,inter-well connectivity testing using chemical tracers is a necessary prerequisite for understanding the characteristics of the water emergence dominant channel and developing recovery enhancement plans.However,common fluorescent tracers are susceptible to interference from fluorescent substances in crude oil in field applications leading to low recognition rate and detection accuracy,which seriously affects the accuracy of test results.In this paper,a combination of 3D fluorescence spectroscopy and parallel factor analysis was used to qualitatively and quantitatively analyze the fluorescent tracer in the simulated recovery fluid under the interference of various fluorescent substances,and the concentration of fluorescent tracer in the simulated recovery fluid was successfully obtained,and the average relative error was calculated to be about 12.75%.The method replaced the traditional fluorescence background test with the data processing of three-dimensional fluorescence spectra,and removed the interference of different fluorescent substances in the simulated extracted fluid with the tracer to be detected,which was characterized by simple operation,high accuracy,low cost,and fast detection,and could be widely used for the rapid detection of fluorescent tracers under complex background conditions.