Study on Rapid Enrichment Method and Activity of Euscaphic Acid in the Fruit of Rosa laevigata Michx
Rosa laevigata Michx.is rich in a kind of pentacyclic triterpenoids,Euscaphic acid(EA),an important natural product which has been widely studied for its diverse pharmacological activities.Due to the low efficiency and long time of traditional separation and purification process,a convenient and quick method is urgently necessary to improve the enrichment efficiency of EA.In this paper,a vacuum column chromatography device was used to enrich EA in the fruits of Rosa laevigata Michx.This method not only saved time and effort,but also greatly improved the enrichment efficiency of EA.Simultaneously,it also provided a real and effective method for the rapid enrichment of other natural active components.In addition,molecular docking of EA revealed that its binding energy to the active site of acetylcholinesterase(AChE)was-36 kJ·mol-1,with a high affinity;The acetylcholinesterase activity of EA was studied and it was found that at mass concentration of 120 μg·mL-1,the inhibitory activity of EA and the positive drug galantamine on AChE reached 83.20%and 92.91%,respectively,indicating that EA has potential anti Alzheimer's disease activity.
Rosa laevigata Michx.Euscaphic acidRapidly enrichmentSelf-designed vacuum deviceAnti-Alzheimer's disease
NETL
NSTL
万方数据
