Study on Highly Stable Liquid D-Allulose 3-Epimerase Enzyme Preparation
The aim of this paper was developing a liquid enzyme preparation of D-allulose 3-epimerase(DAE)with good storage stability.According the half-life evaluations,the key influencing factors were selected using a single factor test.Then the four major influencing factors were selected.Then enzyme preparation formulation was optimized using response surface methodology.The results showed that the optimal compounding process for stable DAE was 0.1 mol.L-1 CaCl2,3.96%trehalose,41.68%glucose,and 29.32%PEG-400.Under these conditions,the half-life was extended to 292 d.The relative error between the simulated value and true value was only 0.17%.This study extended the storage time of DAE through process optimization,significantly improved DAE storage and transportation performance.
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