Structural Characterization and in Vitro Activity of Polysaccharides from Stems and Leaves of Physalis Pubescens L.
The crude polysaccharide was extracted and purified from the stem and leaf of Physalis pubescens L. by water extraction method. A new polysaccharide (PPL-S1) was obtained by Cellulose DEAE-52 and Sephadex G-200 column chromatography. The chemical structure and physicochemical properties were preliminarily explored, and the antioxidant activity and neuroprotective activity of the polysaccharide in vitro were determined. The results showed that the molecular weight of PPL-S1 was 1949.8 Da, and the monosaccharide composition was rhamnose, arabinose, mannose and galactose, and their molar ratio was 0.15:0.21:0.14:0.50. Infrared spectrum analysis showed that it had characteristic absorption peak of polysaccharide. In vitro free radical scavenging experiments showed that PPL-S1 had a certain scavenging effect on DPPH and ABTS free radicals, and the IC50 values were 1.60 mg·mL-1 and 1.43 mg·mL-1, respectively. Moreover, PPL-S1 significantly improved H2O2-induced oxidative stress, improved cell survival rate, and had protective effect on H2O2-induced neuronal injury.
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