Preparation of Resveratrol Thermosensitive Hydrogel and Its Inhibitory Effect on HepG-2 Human Hepatoma Cells
Resveratrol thermosensitive hydrogel was prepared with poloxamer 407 (P407) and poloxamer 188 (P188) as gel matrix. The formulation was optimized by box Behnken design (BBD) method, and its pharmaceutical properties and quality were evaluated. Its inhibitory activity on human hepatoma HepG-2 cells was detected by MTT method. The results showed that the optimal formula was as follows: P40721.22％, P1884.87％, volume ratio of P407 to P1883:1, resveratrol 2.5 mg. The results of electron microscope showed that it had uniform and dense pores, the gelation temperature was 36.4 ℃, the gelation time was 230 s, the pH was 7.12, and the viscosity was 1784.6 mPa·s. The dissolution time was 6 h, the dissolution degree was 95.5％, and the drug release could be more than 90％. The gel system is stable and can release the drug stably. MTT assay showed that it could effectively inhibit the growth of tumor cells in a concentration dependent manner. The prepared resveratrol thermosensitive hydrogel has good thermosensitive effect and can form a local drug repository. It is expected to lay a foundation for future clinical application and achievement transformation.
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