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山楂叶中黄酮和三萜类成分定量分析

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山楂叶作为一种中药材,有着多种活性成分.探讨了以水和不同比例乙醇溶液作为溶剂提取山楂叶的黄酮类和三萜类成分的得率.使用回流提取法,利用不同溶剂对山楂叶进行提取,利用紫外-分光光度计分别对两种溶剂下山楂叶的提取物中的黄酮和三萜类进行含量测定.结果表明:无论是黄酮类还是三萜类,在体积分数为50%乙醇溶液中的提取率最高,且黄酮含量总是大于三萜含量,水提浸膏中的三萜含量较少,推测山楂叶中的三萜类多为苷元结构.通过对山楂叶中黄酮类和三萜类进行含量测定,优化了溶剂选择,为山楂叶活性成分的提取分离提供理论支持.
Quantitative Analysis of Flavonoids and Triterpenoids in Hawthorn Leaves
Hawthorn leaves, as a kind of Chinese medicinal materials, have various active components. In this study, theyield of flavonoids and triterpenoids from Hawthorn leaves was studied under different ratios of ethanol solution and water as solvent. The reflux extraction method was used to extract Hawthorn leaves with different solvents. The contents of flavonoids and triterpenoids in the extracts of Hawthorn leaves were determined by ultraviolet spectrophotometer. The extraction rate of both flavonoids and triterpenes in 50% ethanol was the highest, and the content of flavonoids was always higher than that of triterpenes, but the content of triterpenes in water extract was less, it was speculated that triterpenes in Hawthorn leaves were mostly aglycones. Through the determination of flavonoids and triterpenoids in Hawthorn leaves, the solvent selection was optimized, which provided theoretical support for the extraction and separation of active components in Hawthorn leaves.

Hawthorn leavesFlavonoidsTriterpenoidsSeparationSolvent extraction

史文灏、李卫红、张立新、高品一、李丹琦、刘学贵

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沈阳化工大学 功能分子研究所,辽宁沈阳110142

沈阳化工大学 制药与生物工程学院,辽宁沈阳110142

沈阳化工大学 辽宁省绿色功能分子设计与开发重点实验室,辽宁沈阳110142

沈阳化工大学 硼镁资源开发与精细化工国家地方联合工程实验室,辽宁沈阳110142

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山楂叶 黄酮 三萜 分离 溶剂萃取

辽宁省科技厅项目

2023JH2/101600021

2024

当代化工
中国石油抚顺石化公司,中国石化抚顺石油化工研究院,沈阳市医药和化工行业联合会

当代化工

CSTPCD
影响因子:0.412
ISSN:1671-0460
年,卷(期):2024.53(5)
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