Determination of Six Kinds of Antibiotic Residues in Eggs by High Performance Liquid Chromatography-Tandem Mass Spectrometry
A high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed for the fast detection of six antibiotic residues in eggs. Samples were extracted with acetonitrile, 1％ formic acid acetonitrile and 80％ acetonitrile water (containing 1％ formic acid) respectively. The extract was dried by nitrogen at 40 ℃ and then redissolved with 50％ acetonitrile water (containing 0.1％ formic acid) solution. The samples were separated by C18 column and monitored by Tandem mass spectrometry with electrospray ion in positive and negative ion multi-reaction monitoring mode. The results showed that the recoveries of six antibiotics were higher when 80％ acetonitrile water (containing 1％ formic acid) was used as extraction solvent. Metronidazole, enrofloxacin, ciprofloxacin, tilmicosin and florfenicol showed a good linear relationship between mass concentration and peak area in the mass fraction range of 0.4 ～ 20.0 μg·kg-1, and amoxicillin in the mass fraction range of 4 ～ 200 μg·kg-1, with r > 0.998. The detection limits were between 0.1 μg·kg-1 and 2.0 μg·kg-1. The recoveries were ranged from 85.3％ to 115.0％ with the relative standard deviations of 1.5％ ～ 4.0％ when the spiked levels of metronidazole, enrofloxacin, ciprofloxacin, tilmicosin and florfenicol were 2, 5 and 10 μg·kg-1, and the spiked levels of amoxicillin was 10, 20 and 50 μg·kg-1. The proposed method is simple, fast, low-cost, accurate and reliable,which is suitable for rapid determine of various antibiotics in eggs.
Ultra performance liquid chromatography-tandem mass spectrometryAntibioticsEggs
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