基于活性指导的金樱子果实三萜类化合物分离研究
Isolation of Triterpenoids from Fruit of Rosa laevigata Michx.Based on Activity Guidance
程严 1刘学贵 2李丹琦 2高品一1
作者信息
- 1. 沈阳化工大学制药与生物工程学院,辽宁 沈阳 110142
- 2. 沈阳化工大学功能分子研究所,辽宁 沈阳 110142
- 折叠
摘要
研究了金樱子果实中三萜成分以及抗阿尔茨海默病(AD)的活性.以神经细胞保护活性指导分离,利用硅胶、ODS及反相HPLC等柱色谱方法进行分离纯化,并利用核磁共振波谱方法进行结构鉴定.结果表明:分离的8个三萜类化合物对AChE靶蛋白有高度亲和力,并得到直接靶酶实验验证,其抑制率接近阳性药,表明金樱子三萜类成分具有抗AD活性.
Abstract
The triterpenoids in Rosa laevigata Michx.fruit and their anti-Alzheimer's disease(AD)activities were studied.The neuroprotective was used to guide the isolation.The separation and purification were carried out by silica gel,ODS and reversed-phase HPLC,and the structures were identified by NMR spectroscopy.Eight triterpenoids had high affinity for AChE target proteins,and the inhibition rate was close to that of positive drugs.These results indicated that the triterpenoids had anti-AD activity.
关键词
金樱子/三萜类化合物/活性指导/抗阿尔茨海默病Key words
Rosa laevigata Michx./Triterpenoids/Activity guidance/Anti-Alzheimer's disease引用本文复制引用
基金项目
中共辽宁省委组织部-兴辽英才计划项目(XLYC2007168)
国家自然科学基金项目(81703377)
出版年
2024
NETL
NSTL
万方数据