In order to promote the comprehensive development of new biopolymer materials in China and the high-efficiency application of PHA biomaterial,the optimal extraction process parameters of PHA samples were determined,and the quality and extraction efficiency of PHA products were significantly improved by selecting the type and amount of extractant,the type and amount of pretreatment reagent and the pretreatment time.The results showed that the optimal pretreatment reagent was sodium hypochlorite and the extractant was trichloromethane,and the amounts of both reagents were 14 and 19 times of the mass of PHA sample,respectively,and the best fermentation effect was achieved at a pretreatment time of 1.5 h.The final purity and recovery were 98.35%and 89.60%,respectively.In this study,PHA extraction was carried out using trichloromethane and sodium hypochlorite,which significantly improved the purity and recovery of PHA and simplified the extraction process,providing strong theoretical support in promoting the subsequent separation and recovery of PHA from biomaterials.
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