Isolation and Identification of Activated Sludge Bacteria in Microbial Fuel Cell Systems
The microbial diversity of activated sludge from sewage treatment plants in different regions was analyzed by high-throughput sequencing technology combined with traditional streak plate method.Through different screening media,127 strains of bacteria were selected and cultured,and 12 different microorganisms were obtained by 16S sequencing.According to the species characteristics of microbial taxonomy,the strains were divided into four categories,and microbial colonies were constructed:bacillus,nitrifying bacteria,photosynthetic bacteria and mixed bacteria.The growth curve of the colonies was analyzed by orthogonal experiment.It was found that the growth cycle of the new colonies was about 50 h shorter than that of activated sludge,and the optimal growth temperature of the four colonies was about 30℃.Among them,nitrifying colonies had a wide range of adaptation to pH and could grow normally in the range of pH=5～7,and bacilli and cocci grew fastest in the medium with pH=7.According to the optimized culture conditions and isolated strains,four new types of microbial colonies were constructed to replace the traditional activated sludge and applied to MFC system.
万方数据
维普
