Study on High-Throughput Evaluation Method of Functional Characteristics of Petroleum Degrading Bacteria
Aiming at the problems of cumbersome operation,long cycle and large workload in the traditional petroleum-degrading bacteria degradation performance determination method,the correlation between the degradation rate of petroleum hydrocarbons and four parameters(lipase,dehydrogenase,catechol 2,3-dioxygenase,surfactant)was explored,so as to establish a high-throughput evaluation method for the functional characteristics of petroleum-degrading bacteria.The regression analysis was carried out by SPSS statistical software,and the single-parameter response relationship equation and the multi-parameter fitting equation were obtained and optimized.The results showed that the degradation rate of petroleum hydrocarbons was positively correlated with all parameters,and the emulsification performance of surfactant and the activity of catechol 2,3-dioxygenase were significantly correlated with the degradation rate of petroleum hydrocarbons(R2=0.612 and R2=0.673,P<0.05,respectively).Based on the optimized equation,the method of functional evaluation of petroleum-degrading bacteria by replacing the traditional degradation rate determination method with high-throughput strain enzyme activity determination method is simple,fast and high-throughput,which has broad application prospects in petroleum-degrading strain screening and biota synthesis.
Petroleum hydrocarbon degradationMicrobial remediationEnzyme activityHigh-throughput evaluationMultiple linear regression
NETL
NSTL
万方数据
