Optimization of Extraction Process of Three Antioxidant Components from Rosemary by Orthogonal Test
In order to optimize the extraction conditions of rosmarinic acid,carnosic acid and carnosol from rosemary,and to determine the best extraction technology,the contents of rosmarinic acid,carnosic acid and carnosol in rosemary were determined by high performance liquid chromatography(HPLC).The extraction solvent,solid-liquid ratio and extraction time were selected as the factors.The single factor test combined with L9(34)orthogonal test was used to optimize the extraction process.The results showed that the optimal extraction process was as follows:85%ethanol,1∶10 solid-liquid ratio(g∶mL),60 min extraction time,and repeated extraction twice.The optimized extraction technology can provide reference for the industrial production of three antioxidant components in rosemary.
Orthogonal test methodRosemaryAntioxidant componentsExtraction technology
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