A polypyridine mononuclear nickel metal complex (Ni(acac)2(o-NPIP)](CH3OH)3) was synthesized,the nickel complexes were characterized by spectrum and single crystal diffraction.The binding mode of nickel complex and DNA was revealed by ultraviolet absorption spectrum and fluorescence quenching spectrum.The quenching mechanism,binding constant and action mode of nickel complex and HSA were studied by fluorescence spectroscopy.The results showed that the nickel complexes were six-coordinated mononuclear asymmetric octahedral structures.The nickel complex bonded to DNA by insertion,and the binding constant Kb=1.17×104 L·mol-1.With the addition of nickel complex,the fluorescence intensity of EB-DNA showed a decreasing trend,indicating that the nickel complex competed with EB to replace EB in binding DNA.The apparent bonding constant Kapp=8.03×105 L·mol-1,the nickel complex had moderate bonding DNA capacity.The fluorescence quenching of HSA was caused by the static quenching mechanism of nickel complex,and the binding constant Kb of nickel complex and HSA was 105 orders of magnitude,indicating a strong binding ability.The main binding force between nickel complexes and HSA was the hydrophobic force.Synchronous fluorescence study showed that tryptophan residue was the binding point of nickel complex and HSA.So,the new polypyridine nickel complex synthesized had moderate binding ability to DNA,and could bind to HSA with strong binding ability,and had good biological activity.
维普
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