Study on Screening of Polyacrylamide Fracturing Fluid Degradation Functional Bacteria
Four strains with strong PAM decomposition activity were screened from Tongling red soil and Qinghai plateau soil,named P01,P02,P03 and P04,respectively.P01 was identified as Baciillus cereus,P02 was identified as Bacillus licheniformis,P03 and P04 were identified as Bacills subtillis.At the same time,a functional bacterium capable of degrading PAM was obtained from the oilfield flowback fluid through deep domestication and enrichment screening.It was initially identified as Streptomyces verticillus and named EPAM-01.The effects of pH,temperature,salinity and salinity on the growth of five functional bacteria were investigated.The results showed that the microorganisms grew well at pH=5～9,temperature 30～50℃,salinity 5～12.5 g·L-1,and had a high removal rate of polyacrylamide.Under the same conditions,EPAM-01 was less affected by the environment and could maintain high polyacrylamide degradation activity,and EPAM-01 had strong adaptability to high salinity.
万方数据
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