首页|Knockdown of Microtubule Associated Serine/threonine Kinase Like Expression Inhibits Gastric Cancer Cell Growth and Induces Apoptosis by Activation of ERK1/2 and Inactivation of NF-κB Signaling

Knockdown of Microtubule Associated Serine/threonine Kinase Like Expression Inhibits Gastric Cancer Cell Growth and Induces Apoptosis by Activation of ERK1/2 and Inactivation of NF-κB Signaling

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Microtubule-associated serine/threonine kinase(MASTL)functions to regulate chromosome condensation and mitotic progression.Therefore,aberrant MASTL expression is commonly implicated in various human cancers.This study analyzed MASTL expression in gastric cancer vs.adjacent normal tissue for elucidating the association with clinicopathological data from patients.This work was then extended to investigate the effects of MASTL knockdown on tumor cells in vitro.The level of MASTL expression in gastric cancer tissue was assessed from the UALCAN,GEPIA,and Oncomine online databases.Lentivirus carrying MASTL or negative control shRNA was infected into gastric cancer cells.RT-qPCR,Western blotting,cell viability,cell counting,flow cytometric apoptosis and cell cycle,and colony formation assays were performed.MASTL was upregulated in gastric cancer tissue compared to the adjacent normal tissue,and the MASTL expression was associated with advanced tumor stage,Helicobacter pylori infection and histological subtypes.On the other hand,knockdown of MASTL expression significantly reduced tumor cell viability and proliferation,and arrested cell cycle at G2/M stage but promoted tumor cells to undergo apoptosis.At protein level,knockdown of MASTL expression enhanced levels of cleaved PARP1,cleaved caspase-3,Bax and p-ERKl/2 expression,but downregulated expression levels of BCL-2 and p-NF-κB-p65 protein in AGS and MGC-803 cells.MASTL overexpression in gastric cancer tissue may be associated with gastric cancer development and progression,whereas knockdown of MASTL expression reduces tumor cell proliferation and induces apoptosis.Further study will evaluate MASTL as a potential target of gastric cancer therapeutic strategy.

gastric cancermicrotubule-associated serine/threonine kinasegene expressionshRNA

Cai-xia AN、Shou-pin XIE、Hai-long LI、Yong-hua HU、Rong NIU、Lin-jie ZHANG、Yan JIANG、Qiang LI、Yong-ning Zhou

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Division of Gastroenterology and Hepatology,The First Hospital of Lanzhou University,Lanzhou 730000,China

Department of Neurology,The First People's Hospital of Lanzhou City,Lanzhou 730050,China

Department of Internal Mddicine,The First School of Clinical Medicine,Gansu University of Chinese Medicine,Lanzhou 730000,China

Department of External Chest,Gansu Provincial Cancer Hospital,Lanzhou 730030,China

Division of Pediatric Emergency,Gansu Provincial Maternal and Child Health Hospital,Lanzhou 730050,China

Division of Neurosurgery,The Second Hospital of Lanzhou University,Lanzhou 730000,China

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This study was supported in part by grants from Lanzhou Science and Technology Planning ProjectUniversity Research Project of Gansu ProvinceFoundation of the Fundamental Scientific Research Funds for Colleges and Universities in Gansu ProvinceChina's National Science and Technology Program for Public WellbeingNational Key Research and Development Planning

2016-3-1132018A-049201463-152012GS6201012017YFC0908302

2021

10.1007/s11596-021-2325-2

当代医学科学(英文)
同济医科大学

当代医学科学(英文)

SCI
ISSN：2096-5230
年,卷(期)：2021.41(1)
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