Comparative Study on Chitosan Modified with Different Hepatocyte Ligands as Scaffold Material for Liver Tissue Regeneration:Preparation,Characterization and Evaluation
To achieve the ideal scaffolds for liver tissue regeneration,chitosan(CS)was modified with lactobionic acid(LA)or/and glycyrrhetinic acid(GA)to obtain LA-modified CS(LC),GA-modified CS(GC)and GA/LA-modified CS(GLC),and the composite nanofibrous scaffolds composed of silk fibroin(SF)and the above modified CS were fabricated by green electrospinning.Fourier transform infrared(FTIR)spectroscopy,nuclear magnetic resonance(NMR)spectroscopy and X-ray diffraction(XRD)patterns were used to characterize the chemical components and structures.The water contact angle was measured to evaluate the hydrophilicity,and thermal gravimetric analysis(TGA)was carried out to obtain the thermal properties.These scaffolds were hydrophilic,and their hydrophilicity and thermal stability decreased with the increase of the modified CS content,while their crystallinity increased.The scaffolds showed good performance in promoting the proliferation of the human hepatoma cell line(HepG2 cell)as well as their secretion of both albumin and urea.Furthermore,the scaffolds with LC had a better performance of hepatocellular compatibility than those with GC or GLC.
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