不同肝细胞配体修饰的壳聚糖作为肝组织再生支架材料的比较研究:制备、表征和评价
Comparative Study on Chitosan Modified with Different Hepatocyte Ligands as Scaffold Material for Liver Tissue Regeneration:Preparation,Characterization and Evaluation
王哲 1余凡 1姜玉新 2侯玉婷 1帅兰 1邓敏 2王红声1
作者信息
- 1. 东华大学生物与医学工程学院,上海纳米生物材料与再生医学工程技术研究中心,上海 201620
- 2. 嘉兴大学附属嘉兴市第一医院,嘉兴市病毒相关传染性病重点实验室,浙江嘉兴 314001
- 折叠
摘要
为了获得理想的肝脏再生支架,使用乳酸(lactobionic acid,LA)或/和甘草次酸(glycyrrhetinic acid,GA)对壳聚糖(chitosan,CS)进行改性,得到LA改性的CS(LC)、GA改性的CS(GC)和GA/LA双改性的CS(GLC),并采用绿色静电纺丝法制备了由丝素蛋白(silk fibroin,SF)和上述改性CS组成的复合纳米纤维支架.这些支架是亲水的,其亲水性和热稳定性随着改性CS的增加而降低,而结晶度则相反.这些支架在促进HepG2细胞增殖及分泌白蛋白和尿素方面表现出良好的性能.此外,与SF/GC或SF/GLC支架相比,SF/LC支架具有更好的肝细胞相容性.
Abstract
To achieve the ideal scaffolds for liver tissue regeneration,chitosan(CS)was modified with lactobionic acid(LA)or/and glycyrrhetinic acid(GA)to obtain LA-modified CS(LC),GA-modified CS(GC)and GA/LA-modified CS(GLC),and the composite nanofibrous scaffolds composed of silk fibroin(SF)and the above modified CS were fabricated by green electrospinning.Fourier transform infrared(FTIR)spectroscopy,nuclear magnetic resonance(NMR)spectroscopy and X-ray diffraction(XRD)patterns were used to characterize the chemical components and structures.The water contact angle was measured to evaluate the hydrophilicity,and thermal gravimetric analysis(TGA)was carried out to obtain the thermal properties.These scaffolds were hydrophilic,and their hydrophilicity and thermal stability decreased with the increase of the modified CS content,while their crystallinity increased.The scaffolds showed good performance in promoting the proliferation of the human hepatoma cell line(HepG2 cell)as well as their secretion of both albumin and urea.Furthermore,the scaffolds with LC had a better performance of hepatocellular compatibility than those with GC or GLC.
关键词
甘草次酸(GA)/半乳糖/壳聚糖(CS)/丝素蛋白(SF)/肝组织工程Key words
glycyrrhetinic acid(GA)/galactose/chitosan(CS)/silk fibroin(SF)/liver tissue engineering引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据