Full-length Transcriptome Sequencing and Bioinformatics Analysis of Taxus chinensis var. mairei
In order to fully explore the functional genes and utilize the biological resources of Taxus chinensis var. mairei,PacBio Sequel third-generation sequencing technology was used to conduct high-throughput transcriptome sequencing,and systematic bioinformatics analysis was employed to study the Taxus chinensis var. mairei obtained from Mount Fanjing,Guizhou Province. The study obtained 68.03 Gb of raw data,which included 1001302 raw sequence reading fragments. After assembling,splicing and de-redundancy,65152 unigenes with an average length of 2591 bp were obtained. Database comparison showed that the gene expression of cellular and metabolic activities of the Taxus chinensis var. mairei were higher,indicating strong metabolic activity and genetic information processing capacity. Meanwhile,transcription factors in the transcriptome related to growth and development,stress resistance,secondary metabolism and synthesis provided new insight into the study of the comprehensive utilization and genetic breeding of Taxus chinensis var. mairei.
Taxus chinensis var. maireifull-length transcriptomegene function annotationtranscription factor
万方数据
维普
