To analyze the expression characteristics of CgLOX4 during larval development,the full-length cDNA se-quence of LOX4 was amplified from Pacific oyster(Crassostrea gigas)by using PCR,and the expression character-istics of Cg LOX4 in different stages of larval development were further analyzed by qRT-PCR and whole-mount im-munofluorescence experiments.It was found that the CgLOX4 gene had full-length cDNA sequence of 1 862 bp with an open reading frame of 834 bp encoding 277 amino acids,with the isoelectric point of 8.36.The amino acid se-quence of CgLOX4 gene contained a conserved HOX domain,and CgLOX4 gene was expressed in all the tested tis-sues of adult oyster,with significantly higher expression level in the adductor muscle than that in hepatopancreas,gills,hemolymph,gonad and labial palps tissue(P<0.05).CgLOX4 was expressed in all the examined early em-bryos,with relatively high expression level in fertilized eggs,and four-cells.The whole-mount immunofluorescence assay revealed that the positive signals of LOX4 were mainly distributed in the shell field of trochophore,the viscer-al mass of D-shape larvae and the digestive gland of umbo larvae.The findings indicate that CgLOX4 gene is one of the transcription factors activated earlier in the early development process of the oyster,and may play an important role in regulating the formation of larval shell and digestive organ.
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