摘要
为探究中国花鲈(Lateolabrax maculatus)不同生长阶段肠道和养殖环境微生物组成,选择3月龄(小规格组,体质量为79.89 g±14.97 g)、6月龄(中规格组,体质量为212.67 g±30.32 g)和9月龄(大规格组,体质量为427.56 g±69.56 g)3个不同生长阶段的中国花鲈,采集其肠道微生物和环境微生物(养殖水和池塘底泥)进行16S rDNA基因测序数据分析.结果表明:不同月龄的中国花鲈肠道微生物与环境微生物群落结构有较大差异,而不同生长阶段的环境微生物群落结构则无显著性差异(P＞0.05);环境微生物中双色藻属(Cyanobium_PCC-6307)作为差异物种,表现出与硝酸盐氮呈负相关;肠道微生物分析显示,门水平上,3月龄和6月龄中国花鲈肠道中厚壁菌门(Firmicutes)丰度低于9月龄中国花鲈,属水平上,3月龄和6月龄中国花鲈肠道中富集的可促进糖利用的鲸杆菌属(Cetobacterium)丰度高于9月龄中国花鲈,罗姆布茨菌属(Romboutsia)肠道微生物差异贡献度最高.研究表明,不同养殖阶段的环境微生物群落结构无显著性差异,不同月龄中国花鲈间的肠道微生物群落结构有显著性差异,中国花鲈肠道微生物群落结构受养殖环境的影响.
Abstract
In order to investigate the microbial composition of intestine and aquaculture environment of Chinese sea bass(Lateolabrax maculatus)at different growth stages,16S rDNA gene sequence data were analyzed for microbio-ta in intestine of 3-month-old with body weight of(79.89±14.97)g(small size group),6-month-old with body weight of(212.67±30.32)g(middle size group),and 9-month-old with body weight of(427.56±69.56)g(large size group)Chinese sea bass and aquaculture water and pond sediments.The results showed that there were signifi-cant differences in the structure of intestinal microbial and environmental microbial communities in Chinese sea bass at different ages,while there was no significant difference in environmental microorganisms community structure at different growth stages(P＞0.05).Cyanobium_PCC-6307 as a differential species in environmental microorganisms showed a negatively correlation with nitrate nitrogen levels.Analysis of intestinal microbiota revealed that the abun-dance of Firmicutes was lower in the intestines of 3-month-old and 6-month-old Chinese sea bass than that in 9-month-old Chinese sea bass at the phylum level.At the genus level,there was higher abundance of Cetobacterium promoting sugar utilization in the intestines of 3-month-old and 6-month-old Chinese sea bass than that in 9-month-old Chinese sea bass.There was the maximal differences contribution in Romboutsia at the genus level.The find-ings indicated that there is no significant difference in the microbial community structure of the environment at dif-ferent breeding stages,but there was significant difference in the intestinal microbial community structure among Chinese sea bass at different months of age,the intestinal microbiota of Chinese sea bass was influenced by aqua-culture environmental.
基金项目
广东省重点领域研发计划(2020B0202010009)
广东省普通高等学校创新团队项目(2021KCXTD026)
NETL
NSTL
维普
万方数据