棘皮动物弧菌诱导中间球海胆免疫致敏现象分析
Analysis of immune priming phenomenon in sea urchin(Strongylocentrotus intermedius)induced by Vibrio echinoideorum
殷维骏 1刘岩松 1田文卓 1欧凡江 1刘雷 1丁君 1张伟杰 1常亚青1
作者信息
- 1. 大连海洋大学农业农村部北方海水增养殖重点实验室,辽宁大连 116023
- 折叠
摘要
为探究中间球海胆(Strongylocentrotus intermedins)是否存在免疫致敏现象,使用棘皮动物弧菌(Vibrio echinoideorum)对中间球海胆进行重复感染(首次、二次菌浓度分别为103、104 CFU/mL),检测并比较了两次注射感染后不同时间点中间球海胆体腔细胞密度、吞噬能力、酸性磷酸酶(ACP)活力、活性氧(ROS)含量、总抗氧化能力(T-AOC)等相关免疫指标的变化,以及二次感染后免疫致敏组和对照组间成活率的差异.结果表明:与首次感染相比,二次感染时诱导致敏组的体腔细胞总密度、吞噬细胞密度、ACP、ROS和T-AOC等均出现显著上升(P＜0.05),且响应时间明显缩短,达到峰值的时间分别提前了 36、36、84、12、36 h,且各指标的峰值均显著高于首次感染(P＜0.05);二次感染后,诱导致敏组在6h时的吞噬率(45.41％±6.39％)显著高于对照组(33.17％±1.94％);经过棘皮动物弧菌低浓度诱导后,诱导致敏组存活率(43.33％±10.00％)显著高于未经诱导的阳性对照组(7.78％±10.71％)(P＜0.05).研究表明,低浓度棘皮动物弧菌能诱导中间球海胆产生免疫致敏现象,此结果可为中间球海胆的疾病防控提供新思路.
Abstract
To investigate whether immune priming exists in sea urchin(Strongylocentrotus intermedius),twice in-fection with Vibrio echinoideorum was conducted.Differences in immune parameters such as phagocytic amoebocyte density,red spherule cell density,phagocytic rate,phagocytic index,acid phosphatase activity(ACP),reactive oxygen species(ROS)content,and total antioxidant capacity(T-AOC)between the two time infections were com-pared.Difference in survival rate post challenge between immune priming group and control group were also com-pared.The results showed that the total coelomocytes cell density,phagocyte density,ACP,ROS and T-AOC in the induced immune priming group increased significantly after the second infections(P＜0.05),and the response time to the maximum was 36,36,84,12 and 36 h earlier respectively,and the peak values were significantly higher than those in the first infection(P＜0.05).The phagocytosis rate was significantly higher in the induced priming group(45.41％±6.39％)than that in the control group(33.17％±1.94％)at 6 h post infection.The survival rate was significantly higher in the induced immune priming group(43.33％±10.00％)than that in the uninduced positive control group(7.78％±10.71％)(P＜0.05).The findings indicate that low concentrations of V.echinoideorum can induce immune priming in the sea urchin,which provides new ideas for prevention and control of diseases in sea urchin.
关键词
中间球海胆/棘皮动物弧菌/免疫致敏/免疫指标Key words
Strongylocentrotus intermedius/Vibrio echinoideorum/immune priming/immune parameter引用本文复制引用
基金项目
辽宁省教育厅基本科研面上项目(LJKZ0702)
辽宁省海洋经济发展项目(20220001)
中央财政对辽宁渔业补助项目(2023)()
大连市重点研发计划(2022YF16SN067)
农业部农业科研杰出人才及创新团队项目()
出版年
2024
NETL
NSTL
维普
万方数据