福建省沿海葡萄牙牡蛎遗传多样性和遗传结构分析
Analysis of genetic diversity and genetic structure of Portuguese oyster(Crassostrea angulata)from the coast of Fujian Province
张钰霆 1吴镇 2李聪 2陈火荣 2黄泽坤 3何水清 1张智 1穆景利1
作者信息
- 1. 闽江学院地理与海洋学院,福建福州 350108
- 2. 福建省渔业资源监测中心,福建福州 350003
- 3. 厦门大学海洋与地球学院近海海洋环境科学国家重点实验室,福建厦门 361102
- 折叠
摘要
为研究福建省沿海葡萄牙牡蛎(Crassostrea angulata)天然群体的遗传结构和遗传多样性,根据线粒体CO Ⅰ基因序列对从福建省沿海自然海区(福清、莆田、泉州和东山)采集的373个牡蛎进行鉴定,共发现62个葡萄牙牡蛎,占比16.6％;随后采用GBS(genotyping-by-sequencing)简化基因组测序技术对这4个葡萄牙牡蛎地理群体(福清、莆田、泉州和东山分别为12、12、10、12个)进行遗传多样性、遗传分化及遗传结构分析.结果表明:4个葡萄牙牡蛎群体的观测杂合度(Ho)为0.243～0.271,其中,东山群体的Ho值最高(0.271),福清群体的Ho值最低(0.243);4个群体之间的遗传分化系数(Fst)为0.021 0～0.036 8,其中,东山群体和泉州群体的遗传分化系数最高(0.036 8);遗传结构分析显示,东山群体单独为一个类群,福清、莆田和泉州为另一个类群.研究表明,福建省沿海葡萄牙牡蛎群体的遗传多样性处于较低水平,种质资源有所退化;在种群分化方面,东山群体与福清、莆田和泉州3个群体形成了较明显的遗传分化.
Abstract
In order to investigate genetic diversity and genetic structure of Portuguese oyster(Crassostrea angulata)from the coast of Fujian Province,China,373 samples of the Portuguese oyster were collected from the coastal are-as of Fuqing,Putian,Quanzhou and Dongshan in Fujian Province were sampled,and 62 out of the 373 oyster was identified as C.angulata based on their mitochondrial CO Ⅰ sequences,accounting for 16.6％.Then,the genetic diversity,variation and population structure of these C.angulata were analyzed by the genotyping-by-sequencing(GBS)technology.Results showed that the heterozygosity(Ho)value ranged from 0.243 to 0.271,with the maxi-mal Ho value of 0.271 in the Dongshan population,and the minimal Ho value of 0.243 in the Fuqing population.The Fst value among the 4 populations ranged from 0.021 0 to 0.036 8 with the maximal Fst value of 0.036 8 be-tween the Dongshan and Quanzhou populations.In addition,the result of the genetic structure analysis showed that samples from the Dongshan population were separated from others(the Fuqing,Putian and Quanzhou popula-tions),indicating that there was a low genetic diversity and potential germplasm recession of C.angulata in the coast of Fujian Province and a geographic isolation between the Dongshan population and the other three popula-tions.
关键词
葡萄牙牡蛎/GBS简化基因组测序/遗传多样性/遗传结构Key words
Crassostrea angulata/genotyping-by-sequencing(GBS)/genetic diversity/genetic structure引用本文复制引用
基金项目
福建省科技厅科研项目(2023N0028)
出版年
2024
NETL
NSTL
维普
万方数据