广西北海大风江水域环境及养殖香港牡蛎健康状况分析
Analysis of environment of Dafeng River in Beihai,Guangxi and health status of cultivated Hong Kong oyster(Crassostrea hongkongensis)
刘冉阳 1高磊 2张兴志 3杨文文 2赵俊彦 2赵宝 2于海杰 2许吉祥 2刘璐 2彭金霞 3王玲玲 1宋林生1
作者信息
- 1. 辽宁师范大学 生命科学学院,辽宁 大连 116029;南方海洋科学与工程广东省实验室 (珠海),广东 珠海 519000;大连海洋大学 辽宁省海洋动物免疫学与疫病防控重点实验室,辽宁省海洋动物免疫学重点实验室,辽宁 大连 116023
- 2. 大连海洋大学 辽宁省海洋动物免疫学与疫病防控重点实验室,辽宁省海洋动物免疫学重点实验室,辽宁 大连 116023
- 3. 广西壮族自治区水产科学研究院 广西水产遗传育种与健康养殖重点实验室,广西 南宁 530021
- 折叠
摘要
为了解广西北海大风江水域环境对养殖香港牡蛎(Crassostrea hongkongensis)健康状况的影响,分别在 2023 年 9-10 月进行了 3 次调查研究,并对水体理化环境因子、浮游微藻种类、细菌丰度、香港牡蛎能量储备和氧化应激等指标进行了检测和分析.结果表明:9 月河口水域温度为 28.1℃,盐度为 20.89,水体中总细菌和弧菌丰度分别为 7.22×108、1.49×107cells/L,硅藻丰度较高(约为 1.3×107cells/L),优势种为中肋骨条藻(Skeletonema costatum),养殖香港牡蛎鳃组织中丙二醛(MDA)含量为 9.60 nmol/mg prot,闭壳肌中糖原和葡萄糖含量分别为6.18 mg/g和2.59 μmol/g;10 月河口水域温度下降,盐度上升,水体总细菌和弧菌丰度下降,分别为 4.97×107、1.40×104 cells/L,硅藻丰度下降至 4.3×105cells/L,养殖香港牡蛎鳃组织MDA含量为 1.02 nmol/mg prot,闭壳肌糖原和葡萄糖含量降低至2.49 mg/g和1.94 μmol/g;10 月河口上游水域温度和水体总细菌丰度与同期河口水域相近,但盐度和弧菌丰度均相对较低,硅藻丰度升高,养殖香港牡蛎的闭壳肌糖原和葡萄糖含量升高.研究表明,9 月大风江河口水域养殖的香港牡蛎处于较高的氧化应激状态,可能与高温、高细菌和弧菌丰度等多重环境压力相关;10 月河口水域养殖牡蛎的环境胁迫压力和氧化应激状态有所改善,但饵料藻类丰度的降低导致其糖原含量下降,应对环境变化的抵抗力减弱;10月大风江河口上游水域盐度低、饵料藻丰度高,养殖香港牡蛎处于相对健康状态.
Abstract
To elucidate the impacts of water environment of Dafeng River in Beihai,Guangxi,on health status of Hong Kong oyster(Crassostrea hongkongensis)cultured therein,the physicochemical factors in water quality,planktonic microalgae,bacterial abundance,and energy reserve and oxidative stress of Hong Kong oysters were sur-veyed in the main Hong Kong oyster farming area of Beihai Sea of Guangzhou,the mouth of the Dafeng River and the upper waters(108°52′49″E,21°38′26″N;108°51′30″E,21°46′22″N)in September and October 2023.The results showed that,the estuarine water temperature was 28.1℃,with a salinity of 20.89,total bacterial abun-dance of 7.22×108 cells/L and Vibrio abundance of 1.49×107 cells/L in September.Diatom abundance was found to be approximately 1.3×107 cells/L,with the dominant species of Skeletonema costatum.The malondialdehyde(MDA)content of 9.60 nmol/mg protein was observed in the gill of cultivated Hong Kong oysters,with glycogen content of 6.18 mg/g and glucose content of 2.59 μmol/g in the adductor muscle.In October,however,the estuar-ine water temperature was decreased to 25.7℃,accompanied by an increase in salinity to 25.50,with bacterial a-bundance decreased to 4.97×107 cells/L and Vibrio abundance to 1.40×104 cells/L and diatom abundance de-creased to 4.3×105 cells/L in the water.MDA content was 1.02 nmol/mg protein in the gill of cultivated Hong Kong oyster,and glycogen and glucose content in the adductor muscle was decreased to 2.49 mg/g and 1.94 μmol/g,re-spectively.The water temperature and total bacterial abundance were similar to those in the estuarine waters in the upstream waters in October.However,salinity and Vibrio abundance were relatively low,with high diatom abun-dance.The glycogen and glucose contents in adductor muscle were increased in cultivated Hong Kong oysters.In summary,Hong Kong oysters cultured in the Dafeng River estuary faced multiple environmental stressors,including high temperature,elevated bacterial and Vibrio abundances in September,leading to increased oxidative stress.In October,these oysters primarily encountered a single environmental pressure,high salinity,and improved oxidative stress status.The decrease in algal food led to decline in glycogen content and resistance to environmental changes in October,and there were lower salinity,higher diatom abundance,and relatively healthier conditions for Hong Kong oyster cultivation in upstream waters in the Dafeng River.
关键词
香港牡蛎/大风江/水环境/能量储备/氧化应激Key words
Crassostrea hongkongensis/Dafeng River/water environment/energy reserve/oxidative stress引用本文复制引用
基金项目
南方海洋科学与工程广东省实验室(珠海)资助项目(SML2023SP237)
国家自然科学基金(41961124009)
国家自然科学基金(32320110)
财政部和农业农村部:国家现代农业产业技术体系资助(CARS-49)
农业农村部农业科研杰出人才及其创新团队项目()
出版年
2024
NETL
NSTL
万方数据