In order to explore the low-temperature preservation and transportation technology of freshwater fish,se-rum biochemical indices and contents antioxidant index were detected in grass carp(Ctenopharyngodon idellus)with body weight of(1 200±100)g at water temperature from 23-25℃(control group)to 24,20,18,16,14,and 12℃at cooling rate of 3℃/h at>25℃,10℃at≤25℃,and 2℃/h at≤10℃and at salinity of 0.5-5 for 48 h and total ammonia nitrogen concentration in water was determined.The results showed that the grass carp had critical temperature of 6-8℃,and semi-dormant temperature of 16℃at salinity of less than 3.The grass carp with delayed stress at 16℃and lower concentration of ammonia and nitrogen in the water column had the maximal survival rate,lower serum levels of AST,ALT,COR and GLU at 48 h of preservation,whereas the maxi-mal enzyme activities of SOD,CAT,and GSH-Px were found,with significantly lower content of MDA than that in other groups(P<0.05).Lower ammonia nitrogen concentrations in the water column,the maximal survival rate of grass carp,generally lower levels of the biochemical indicators in the serum of grass carp,higher activities of an-tioxidant enzymes and lower levels of MDA were observed at salinity from 1 to 2 for 48 h of preservation.The Pear-son and Mantle tests revealed that the effects of temperature on COR,GLU and GSH-Px,and salinity on ALT,AST,COR,GLU and SOD were more significant.The water quality was improved and long-distance transport of grass carp with water was facilitated at water temperature of 16℃and appropriate salinity.The key parameters of the transient conditions of grass carp in this experiment provide technical support for the live transport of bulk fresh-water fishes and reference for the development of relevant transient and transport.
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