克氏原螯虾3群体双列杂交群体耐高温性能比较
Comparison of high temperature tolerance in three population diallel hybrid population of red swamp crayfish(Procambarus clarkii)
吴玮杰 1徐啟洲 1万通 1贾亮 1程熙 2李典中 3白志毅4
作者信息
- 1. 上海海洋大学 农业农村部淡水水产种质资源重点实验室,上海 201306
- 2. 上海市嘉定区水产技术推广站,上海 201800
- 3. 芜湖盛典休闲生态园有限公司,安徽 芜湖 241212
- 4. 上海海洋大学 农业农村部淡水水产种质资源重点实验室,上海 201306;上海海洋大学 上海市水产动物良种创制与绿色养殖协同创新中心,上海 201306
- 折叠
摘要
为构建耐高温性状的克氏原螯虾(Procambarus clarkii)杂交群体,以芜湖、建湖、滆湖 3 个克氏原螯虾群体完全双列杂交的 9 个F1群体为亲本,通过自交获得F2群体[滆湖♀×建湖♂(GJ)、建湖♀×滆湖♂(JG)、滆湖♀×芜湖♂(GW)、芜湖♀×滆湖♂(WG)、建湖♀×芜湖♂(JW)、芜湖♀×建湖♂(WJ)、滆湖♀×滆湖♂(GG)、建湖♀×建湖♂(JJ)、芜湖♀×芜湖♂(WW)],采用耐热性(upper thermal tolerance,UTT)、心率的阿伦尼乌斯拐点温度(arrhenius break temperatures,ABT)作为评定指标,并结合临界温度法(critical thermal methodology,CTM)、半致死时间(half leathaltime,LT50)等方法,比较了 9 个F2群体对高温的耐受性,并进一步分析了克氏原螯虾UTT值、ABT值与体质量等性状的相关性.结果表明:各群体UTT值排序为GW>GJ>WJ>JG>GG>WW>WG>JW>JJ,且GW群体UTT值显著高于其余群体(P<0.05);各群体ABT排序为GW>WJ>WW>WG>JW>JJ>JG>GJ>GG,且GW群体ABT显著高于其余群体(P<0.05);各群体CTMax排序为GW>WJ>GJ>GG>WW>JG>JW>WG>JJ,且GW、WJ、GJ群体显著高于其余群体(P<0.05);各群体LT50排序为GW>GJ>WJ>JG>WW>GG>JW=JJ>WG;不同规格克氏原螯虾个体之间耐热性(UTT)存在显著性差异(P<0.05),即克氏原螯虾虾苗耐热性显著高于初达上市规格的商品虾(P<0.05);克氏原螯虾热胁迫前后心率均与体质量呈负相关,相关系数分别为-0.21 和-0.15(P<0.05).研究表明,滆湖♀×芜湖♂(GW)群体 4 个耐高温指标均优于其他群体,与UTT等指标相比,采用ABT指标测定对个体损害小,更适用于良种选育.
Abstract
In order to establish a hybrid population of red swamp crayfish(Procambarus clarkii)with high tempera-ture tolerance,nine F1 populations of complete diallel hybridization from three populations of red swamp crayfish,Wuhu,Jianhu,and Gehu,were used as broodstocks to obtain F2 populations through self pollination[Gehu♀×Jianhu♂(GJ),Jianhu♀×Gehu♂(JG),Gehu♀×Wuhu♂(GW),Wuhu♀×Gehu♂(WG),Jianhu♀×Wuhu♂(JW),Wuhu♀×Jianhu♂(WJ),Gehu♀×Gehu♂(GG),Jianhu♀×Jianhu♂(JJ),and Wuhu♀×Wuhu♂(WW)]exposed to the water temperature from 30℃to 35℃at raising rate of 1℃/2 h,and at raising rate of 0.5℃/4 h until the crayfish death.The tolerance of 9 F2 populations to high temperature was compared using evalua-tion indicators of upper thermal tolerance(UTT)and Arrhenius Break Temperatures(ABT)of heart rate,com-bined with Critical Thermal Methodology(CTM),median lethal Time(LT50)and other methods,and the correla-tion was analyzed between ABT values and traits including body weight.The results showed that the descending or-der of the UTT values in each group were ranked as GW>GJ>WJ>JG>GG>WW>WG>JW>JJ,with significantly higher in the GW group than that in the other groups(P<0.05).The ABT was expressed as GW>WJ>WW>WG>JW>JJ>JG>GJ>GG in each group,significantly higher in the GW group than that in the other groups(P<0.05).The CTMax was found to be GW>WJ>GJ>GG>WW>JG>JW>WG>JJ in different groups,significantly higher in the GW,WJ,and GJ groups than that in the other groups(P<0.05).The LT50 was described as GW>GJ>WJ>JG>WW>GG>JW=JJ>WG in the red swamp crayfish in each group.There was significant difference in heat tolerance(UTT)among individuals in the crayfish with different developmental stages(P<0.05),significantly higher heat tolerance in crayfish juveniles than that in remarkable crayfish just reached the market specifications(P<0.05).Before and after heat stress,there was a negative correlation between heart rate and body weight in crayfish,with correlation coefficients of-0.21 and-0.15,respectively(P<0.05).The(GW)population had superior to other populations in four high temperature tolerance indicators.Compared with UTT and other indicators,using ABT indi-cators to measure was less harmful to individuals and more suitable for breeding high-quality varieties.
关键词
克氏原螯虾/耐高温性能/耐热性/心率拐点温度/群体Key words
Procambarus clarkii/high temperature resistance performance/upper thermal tolerance(UTT)/ar-rhenius break temperatures(ABT)/population引用本文复制引用
基金项目
上海市科技创新行动计划项目(23N11900300)
安徽省水产种业振兴项目(2022AHNYNC081FW)
出版年
2024
NETL
NSTL
万方数据