To explore the function of the calcium-binding protein S100P gene(On S100P)in Nile tilapia(Oreo-chromis niloticus),the On S100P gene was cloned through specific primers designed according to the On S100P se-quence obtained from the transcriptome data of tilapia head kidney.The tissue distribution and the expression pro-files of On S100P in various tissues of the Nile tilapia exposed to Poly(I∶C)stimulation were examined by real-time fluorescence quantitative PCR.The recombinant protein of On S100P was expressed and prepared,and was used to incubate Nile tilapia head kidney-derived lymphocytes for the determination of regulatory roles in inflamma-tory factors expression.The results showed that the length of the CDS of On S100P gene was 288 bp,encoding 96 a-mino acids and possessing a conserved S100 domain of S100 protein family.Phylogenetic tree analysis revealed that On S100P had the relative high homology with Maylandia zebra S100P.On S100P was expressed in different tissues of Nile tilapia,with the maximal levels in muscle and gill.After Poly(I∶C)stimulation,the expression levels of On S100P in the intestinal,head and kidney and brain of tilapia were significantly up-regulated,with the peak at 24 h post stimulation.The obtained On S100P recombinant protein(r On S100P)induced the expression of MIF,IL8,TNF-β and IL1-β in head kidney-derived lymphocytes.The findings indicated that On S100P had a conserved S100 domain,similar biological functions like other S100 protein family,and participated in the fish innate immunity by modulating the inflammatory factors expression of lymphocytes.
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