In order to investigate the toxic chronic effects of tetrabromodiphenyl ether(BDE-47)on juvenile mar-bled flounder(Pseudopleuronectes yokohamae),the juvenile marbled flounder with body weight of 1.31 g±0.03 g were kept in a culture tank containing 40 L of water and exposed to blank control(CK),solvent control group(DMSO),0.25(LC),0.5(MC),and 2.0 mg/L BDE-47(HC)at water temperature of(18.4±0.85)℃ by semi-static water contact exposure method,and the morphological changes of gill tissue were histologically observed in the juvenile marbled flounder exposed to BDE-47 stress.The molecular mechanism of gill tissue under BDE-47 stress was analyzed by miRNA sequencing and mRNA sequencing.The results showed that swelling,shedding and hyperplasia repair,and significant increase in mucus cells were observed in the epithelial cells of gill lamellae of the marbled flounder exposed to BDE-47 stress,more severe with the elevated BDE-47 concentration.Transcrip-tome data showed that changes in the expression of ErbB signaling pathway genes caused the proliferation and repair of gill lamellae epithelial cells.miRNA sequencing analysis revealed that the shedding of gill lamellae epithelial cells caused by BDE-47 exposure was involved in MAPK and Ras signaling pathways.The lipoic acid metabolism pathway and Toll-like receptor signaling pathway were primarily in the combined analysis of mRNA and miRNA,speculating that miRNAs in gill tissue affected the toxic effect of BDE-47 on the gills of marbled flounder in water by regulating the stability and translation of lipoic acid metabolism and Toll-like receptor signaling pathway gene mRNA.The findings indicate that chronic stress with different concentrations of BDE-47 can cause different degrees of damage to the gill tissue structure of marbled flounder.
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