In order to evaluate the transport and transformation of antibiotics in marine fish and evaluate the ecolog-ical risk of antibiotics,the bioconcentration pattern and metabolic pathway of sulfamethazine(SMZ)were detected in rockfish Sebastes schlegelii reared in a 30 L container and exposed to 0(control group),10,and 100 μg/L SMZ at water temperature of(20±0.5)℃ by liquid chromatography tandem mass spectrometry quantitative analysis and high-resolution mass spectrometry detection and analysis.The results showed that the maximal enrichment concen-tration of SMZ was observed in the fish exposed to SMZ in the third day during the whole exposure period,without significant difference between the maximum concentration of SMZ and the high and low concentrations of SMZ in fish.During the optimal enrichment period,the enrichment trend of SMZ in the fish was described as gill＞muscle＞liver.Metabolic analysis showed that the metabolites of SMZ in the test fish were N4-acetylated SMZ and 2-amino-4,6-dimethylpyrimidine.The main metabolic pathways were SMZ acetylation addition and N-S bond cleavage linked to 2-amino-4,6-dimethylpyrimidine.In conclusion,sulfonamides antibiotics can migrate to the main tissues and or-gans of marine fish,and the main metabolic pathways of SMZ in Sebastes schlegelii are acetylation addition and N-S bond breakage.
万方数据
维普
