To explore the nutrient quality differences and status of gut microbiota in Chinese mitten handed crab(Eriocheir sinensis)from the cold regions of northern China after a long period of natural overwintering in low-tem-perature environments,tissue indicators,muscle nutritional components,mineral elements,and fatty acid content were analyzed in the crab with body weight from 104 g to 140 g,and the composition of gut microbiota in the crabs collected from Xiangyang Lake(47° 9′36″N,88° 7′12″E)and(47°8′24″N,88°10′12″E)of Xinjiang Produc-tion and Construction Corps before and after overwintering by high-throughput sequencing technology of the 16S rRNA.The results showed that the condition factor and hepatopancreas index were found to be significantly de-creased in the female crab(P＜0.05),while the condition factor and gonadosomatic index to be significantly in-creased in the male crab(P＜0.05),with significant change in the contents of mineral elements Na,K,Mn,Fe and Cu in different trends(P＜0.05),and significant increase in the content of unsaturated fatty acidsy(P＜0.05)after natural overwintering in the lake.The diversity and abundance of the gut microbiota were shown to be de-creased in crab after overwintering,with noticeable changes in microbial composition.Firmicutes(71.14％),Bacteroidota(15.19％),and Proteobacteria(9.69％)were the dominant phyla before overwintering,and Pro-teobacteria(52.79％),Firmicutes(37.10％),and Bacteroidota(4.35％)after overwintering.The abundance of the genus Psychrobacter within Proteobacteria was increased significantly from 0.10％before overwintering to 43.75％,becoming the dominant genus,after overwintering.The findings indicate that the quality of crab does not significantly decline after a long period of natural overwintering in Xinjiang Uygur Autonomous Region,and some nutritional indicators are better than before overwintering,and the structure of gut microbiota also undergoes adap-tive adjustments.These findings provide basis for exploring new cultures and sales models of E.sinensis in this re-gion.
维普
万方数据