In order to investigate the effects of temperature and light on the short-term interaction of photosynthesis in green laver(Enteromotpha prolifera)sampled at coastal Qingdao(120°11′24″E,36°2′24″N),three tempera-ture(4 ℃,20 ℃,and 30 ℃)and three light intensities(20,140,and 400 μmol/(m2·s))interaction were e-valuated,and the photosynthetic fluorescence parameters and differential gene expression were analyzed in the green laver treated for 24 h.The results showed that there was synergistic stress effect of short term high temperature and high light on the alga during the test cycle.The photochemical efficiency(Fv/Fm)of the alga was shown to be significantly reduced by high temperature and high light,with the down-regulated genes of the light trapping com-plex in high temperature and high light environments,and up-regulated in low temperature and low light.The alga attenuated the adverse environmental effects on the photosystems through up-regulation of the genes of the D1 pro-tein,the CP43 protein and the manganese stabilising protein in the high temperature/high light and the low temper-ature/low light conditions,attenuating the negative regulatory effects of unfavourable environments on photosystemⅡ.The expression of genes encoding enzymes related to the C3 pathway was found to be down-regulated and the ex-pression of genes related to the C4 pathway phosphoenolpyruvate carboxylase was up-regulated in high temperature and high light,effectively segregating O2 binding to ribulose-1,5-bisphosphate carboxylase/oxygenase,essentially eliminating photorespiration.The C4 pathway was the result of seaweed's evolution to reduce the damage of photore-spiration.It was shown that green laver was able to tolerate changes in low or high temperatures(4℃,30 ℃)as well as low and high light[140 μmol/(m2·s),400 μmol/(m2·s)],and withstanded environmental stresses through a variety of mechanisms.The findings may provide a useful reference for exploring the molecular mecha-nisms of green tide outbreaks in the Yellow Sea.
维普
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