Evaluation of the genetic diversity and genetic structure of five populations of snakehead(Channa argus)using SLAF-seq
In order to probe into the genetic structure and genetic diversity of local populations of snakehead(Channa argus),the population genetic structure and genetic diversity were analyzed in five populations of snake-head collected from Fuyuan(HLJ)in Heilongjiang province,Neijiang(NJH and NJB)in Sichuan Province,Weishan Lake(SD)in Shandong province,and Huzhou(ZJ)in Zhejiang Province from April 2017 to September 2018.A total of 1 987 293 population SNP markers were detected,with an average sequencing depth of 16.33 x,and average Q20 content of 99.17%and average GC content of 41.19%.It was found that the observed heterozygos-ity(Ho)was ranged from 0.078 to 0.134,the expected heterozygosity(He)ranged from 0.094 to 0.183,the con-tent of polymorphic information from 0.156 to 0.250,the inbreeding coefficient(FIS)from 0.288 to 0.579,and the nucleotide polymorphisms(π)from 2.96×10-6 to 5.73×10-6 in the genetic diversity of the five populations.The ge-netic differentiation and genetic structure analyses revealed that the gene flow(Nm)among the populations was ranged from 0.503 to 0.856,less than 1.The coefficient of genetic differentiation(Fst)among the populations was found to be from 0.042 to 0.247.Phylogenetic tree showed that the populations of HLJ,SD,and ZJ were clustered into a single unit,and that NJH and NJB were clustered into a single unit.The five populations were classified into four subpopulations,except for ZJ population,with gene exchange from ancestral subpopulations in other regional groups.The identification and genotyping of the genetic diversity of the snakehead populations were realized at a low cost by SLAF-seq technology,with lower Ho than the He in all populations,and certain degree of inbreeding in the populations.There were low heterozygosity,polymorphic information content and nucleotide polymorphisms in the five populations,indicating a low level of genetic diversity,and the differences in nucleotide polymorphisms among the small populations.There were different genetic differentiation levels in the five populations,with NJH and NJB at a low level of genetic differentiation,and medium or high level of genetic differentiation in the rest of popula-tions,forming a more obvious and distinctive pattern.The five C.argus populations had different levels of genetic differentiation.
万方数据
维普
