To clarify the genetic background of different cultured populations of Pangasianodon hypophthalmus,10 microsatellite markers with high amplification efficiency and specificity were identified by genetic diversity analysis,and used to analyze the genetic diversity of four cultivation populations from Guangzhou,Zhanjiang,and Foshan(Guangdong province),and Wenchang(Hainan province).The results showed that the 10 microsatellite loci ex-hibited high polymorphism,with an average polymorphic information content(PIC)of 0.702.The 10 primer pairs detected a total of 74 alleles(Na)within the cultivation populations.The observed heterozygosity(Ho)was found to be ranged from 0.482(Pab013)to 0.841(Pab040),and the expected heterozygosity(He)from 0.555(Pab013)to 0.883(Pab063).Based on genetic distances,the four cultured groups were divided into two genetic populations,Hainan group forming its own branch,and the other three populations clustering together,with the most closely related in Guangzhou and Foshan populations.The maximal genetic distance between groups(0.475 686)was observed between Wenchang population in Hainan province and Zhanjiang population in Guang-dong province,and the minimal(0.116 786)between Guangzhou in Guangdong province and Foshan population in Guangdong province.It was found that the four cultured populations have a medium to high level of genetic diversi-ty,showing potential for further selective breeding.The Hainan population exhibited significant genetic differentia-tion from the other three populations,enriching the genetic resources of P.hypophthalmus.The finding provides es-sential data for the conservation and scientific management of P.hypophthalmus aquaculture genetic resources.
万方数据
维普
