Objective To compare the behavioral differences in Parkinson's disease (PD) rat models with 6-hydroxydopamine lesions of the unilateral striatum and the unilateral medial forebrain bundle (MFB) after deep brain stimulation of the subthalamic nucleus (STN-DBS) with different current intensities.Methods Twenty-four adult male Sprague-Dawley (SD) rats were randomly divided into two groups, 1-site striatum lesion model group (n=12) and MFB lesion model group (n=12).Both groups were treated with STN-DBS at incrementally increasing current intensities of 0, 100, 150 and 200 μA.The differences in behavioral performance were compared using methamphetamine-induced rotational test, walking test and rotarod test.Results During methamphetamine-induced rotational test, when the current intensity reached 200 μA, the rotation count of the MFB lesion group decreased to (0.7±0.5) r/min, while the rotation of the 1-site striatum lesion group completely stopped (0 r/min).Compared to stimulation at 150 μA, the rotation symptoms of both groups were significantly improved (P<0.01).During walking test and rotarod test, when the current intensity reached 150 μA, compared to 100 μA stimulation, the initiation time in the 1-site striatum lesion group and the MFB lesion group significantly shortened to (4.2±1.8) s and (18±2.3) s, respectively.The average step length significantly increased to (14.6±0.6) cm and (14.8±0.8) cm, respectively.The number of step adjustments (forward) increased significantly to (4.5±0.5) and (4.2±0.4) steps, respectively.Step adjustments (backward) increased significantly to (11.2±0.3) and (11.4±0.4) steps, respectively.The ORP of rotarod test increased to (3405±572) and (2904±931) scores, respectively.The symptoms of movement disorders were significantly improved (P<0.01).Conclusion The electrical stimulation parameters for obtaining significant therapeutic effects in the STN-DBS treatment experiments on PD rat models are different for different symptoms.According to different experimental objectives, appropriate behavioral experimental methods should be selected to screen the best electrical stimulation parameters.
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