Diagnostic and prognostic value of serum B7-H3 combined with tumor M2 type pyruvate kinase in lung cancer
Objective To study the diagnostic and prognostic value of serum B7-H3 and tumor M2 type pyruvate kinase (TuM2-PK) in lung cancer.Methods From June 2020 to June 2021, 137 patients with lung cancer and benign lung lesions admitted to our department were selected.Among them, 85 patients with lung cancer were divided into the experimental group, and 52 patients with benign lung lesions were divided into the control group.ELISA was used to measure the serum levels of B7-H3 and TuM2-PK, which were compared across different patient groups based on lymph node metastasis, staging, differentiation degree, and pathological types.ROC curves were plotted to evaluate the diagnostic value of B7-H3 and TuM2-PK for lung cancer.The DeLong method was used to measure the area under the ROC curve (AUC) of each indicator.Over a 10-month follow-up period, COX regression analysis was used to investigate the impact of B7-H3 and TuM2-PK on disease progression.Spearman correlation was used to analyze the relationship between serum B7-H3 and TuM2-PK levels and progression free survival (PFS).Results The serum levels of B7-H3 and TuM2-PK in the experimental group were (31.45±6.64) ng/mL and (27.84±7.09) U/mL, respectively, which were higher than those in the control group [(21.11±7.44) ng/mL and (18.88±6.63) U/mL, P<0.01].The serum levels of B7-H3 and TuM2-PK in patients with lymph node metastasis, stage Ⅲ-Ⅳ, and moderate-to-poor differentiation were higher than those without lymph node metastasis, stage Ⅰ-Ⅱ, and well differentiation.COX regression analysis indicated that the serum levels of B7-H3 and TuM2-PK in lung cancer patients were independent risk factors (P<0.05) and correlated with disease progression.Spearman correlation analysis showed that serum B7-H3 and TuM2-PK levels were negatively correlated with their PFS (r=-0.806, r=-0.723, P<0.001).The analysis results of the ROC curve showed that the sensitivity of B7-H3, TuM2-PK, and their combined diagnosis were 76.5%, 75.3%, and 84.7%, respectively, and the specificity were 76.9%, 76.9%, and 78.8%, respectively.The AUC were 0.842 (95%CI 0.776-0.908), 0.822 (95%CI 0.751-0.893), and 0.901 (95%CI 0.850-0.953), respectively.The critical values of B7-H3 and TuM2-PK in serum were 26.51 ng/mL and 25.12 U/mL, respectively.The DeLong test revealed that the combined diagnosis had better predictive value for the diagnosis of lung cancer than either marker alone (P<0.05).Conclusion The combined diagnostic value of B7-H3 and TuM2-PK in serum for lung cancer is higher than either marker alone, and both markers have certain predictive effect on disease progression and pathological type.
lung cancerB7-H3tumor M2 type pyruvate kinaseclinical pathologydiagnostic value
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维普
