Vertical Distribution of Methane-Metabolizing Microorganisms and Molecular Ecological Networks in Dajiuhu Peatland,Shennongjia
Soil samples,at the depth of 0 cm,10 cm,30 cm,and 50 cm,were collected from the Dajiuhu peatland for four quarters in 2019 to investigate the distribution patterns of methanogens and methanotrophs in vertical profile and their response to the abiotic environment.High-through put sequencing was implemented to analyze the community structure of methanogens and methanotrophs,then construct molecular ecological networks.Methanoregula and Methylocapsa were found as the largest dominant genus of methanogens and methanotrophs in the Dajiuhu peatland.The ecological networks of methanogens and methanotrophs and their synergism varied with depth,and the opposite situation was observed in methanogens and methanogens.Moreover,Methanoregula,Methanocella,Methanobacterium were identified as key species in the methanogenic community,and 4 key species of methanotrophs were Methylocapsa,Candidatus Methyloumidiphilus,Methylocystis,Candidatus Methylospira.The results demonstrated that total carbon,total nitrogen,ammonium,and nitrite could affect the structure of methanogens and methanogens,thereby controlling CH4 emissions in the Dajiuhu peatland.