摘要
为了探讨产甲烷菌和甲烷氧化菌群落随土壤深度的垂直分布特征及甲烷代谢菌对环境的响应机制,于2019年4个季度采集大九湖泥炭地0 cm、10 cm、30 cm和50 cm的土壤样品.通过功能基因高通量测序获得产甲烷菌和甲烷氧化菌群落结构组成并构建分子生态网络.产甲烷菌和甲烷氧化菌的最大优势属分别为Methanoregula和Methylocapsa;产甲烷菌和甲烷氧化菌群落生态网络结构及其共现模式随深度发生变化,产甲烷菌和甲烷氧化菌群落结构随深度呈相反的变化趋势;产甲烷菌和甲烷氧化菌网络中关键物种分别有3个和4个,分别为Methanoregula、Methanocella、Methano bacterium和Methylocapsa、Candidatus Methyloumidiphilus、Methylocystis、Candidatus Methylospira.表明环境因子土壤总碳、总氮、铵态氮和亚硝态氮含量等环境因子通过影响产甲烷菌和甲烷氧化菌群落进而改变CH4排放.
Abstract
Soil samples,at the depth of 0 cm,10 cm,30 cm,and 50 cm,were collected from the Dajiuhu peatland for four quarters in 2019 to investigate the distribution patterns of methanogens and methanotrophs in vertical profile and their response to the abiotic environment.High-through put sequencing was implemented to analyze the community structure of methanogens and methanotrophs,then construct molecular ecological networks.Methanoregula and Methylocapsa were found as the largest dominant genus of methanogens and methanotrophs in the Dajiuhu peatland.The ecological networks of methanogens and methanotrophs and their synergism varied with depth,and the opposite situation was observed in methanogens and methanogens.Moreover,Methanoregula,Methanocella,Methanobacterium were identified as key species in the methanogenic community,and 4 key species of methanotrophs were Methylocapsa,Candidatus Methyloumidiphilus,Methylocystis,Candidatus Methylospira.The results demonstrated that total carbon,total nitrogen,ammonium,and nitrite could affect the structure of methanogens and methanogens,thereby controlling CH4 emissions in the Dajiuhu peatland.
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