Localization and clone of four segments of highly conserved sequence of βig-h3 gene and construction of their prokaryotic expression vector
AIM: To locate the four segments of highly conserved sequence of the βig-h3,to clone the total gene of βig-h3 and its four segments of highly conserved sequence, and to construct their prokaryotic expression vectors respectively. METHODS: The total gene of βig-h3 and its four segments of highly conserved sequence were amplified by RT-PCR and cloned into pRSET-A vector. The recombinant plasmids for prokaryotic expression were constructed respectively. RESULTS: The results of sequence analysis demonstrated that the total gene of βig-h3 and its four segments of highly conserved sequence were completely consistent with the sequence reported in GenBank and they were correctly cloned into prokaryotic expression vector respectively. CONCLUSION: The prokaryotic expression vectors with target genes are constructed successfully, which provides fundamental proof for further study of the structural and functional relationship of the βig-h3.
NETL
NSTL
万方数据
维普
