βig-h3基因4段高度保守序列定位、克隆及原核表达载体的构建
Localization and clone of four segments of highly conserved sequence of βig-h3 gene and construction of their prokaryotic expression vector
戴竞耀 1唐娟 2蒋建利 2窦科峰 3陈志南2
作者信息
- 1. 第四军医大学细胞工程研究中心;第四军医大学西京医院肝胆外科
- 2. 第四军医大学细胞工程研究中心
- 3. 第四军医大学西京医院肝胆外科
- 折叠
摘要
目的:确定βig-h3基因4段高度保守序列的序列位置,克隆βig-h3全长及4段高度保守序列的基因,分别构建原核表达载体.方法:用RT-PCR方法获得βig-h3全长及4段高度保守序列的基因,以pRSET-A为载体,分别构建重组原核表达质粒.结果:测序结果证实获得βig-h3全长及4段高度保守序列的基因,其序列与GenBank中报道完全一致.分别正确连入pRSET-A载体中,读码框正确.结论:成功构建βig-h3基因全长及4段高度保守序列的原核表达载体,为进行结构与功能相关性研究奠定了基础.
Abstract
AIM: To locate the four segments of highly conserved sequence of the βig-h3,to clone the total gene of βig-h3 and its four segments of highly conserved sequence, and to construct their prokaryotic expression vectors respectively. METHODS: The total gene of βig-h3 and its four segments of highly conserved sequence were amplified by RT-PCR and cloned into pRSET-A vector. The recombinant plasmids for prokaryotic expression were constructed respectively. RESULTS: The results of sequence analysis demonstrated that the total gene of βig-h3 and its four segments of highly conserved sequence were completely consistent with the sequence reported in GenBank and they were correctly cloned into prokaryotic expression vector respectively. CONCLUSION: The prokaryotic expression vectors with target genes are constructed successfully, which provides fundamental proof for further study of the structural and functional relationship of the βig-h3.
关键词
βig-h3/高度保守序列/原核表达载体Key words
βig-h3/highly conserved sequence/prokaryotic expression vector引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据