Comparison of inhibitive effects of Curcumin and Calebin-A on human hepatoma cell line HepG2
AIM: To compare the inhibitive effects of Curcumin and Calebin-A on proliferation of human hepatoma cell line HepG2 cells. METHODS: MTT assay was used to evaluate the IC50 of Curcumin and Calebin-A on the cells. Colony formation assay was used to detect the ability of cell proliferation, when exposed to Curcumin and Calebin-A at 25 μmol/L. Cell apoptosis induced by two drugs was analyzed by TUNEL assay. RESULTS: Both Curcumin and Calebin-A inhibited the growth of HepG2 cells in a dose- and time-dependent manner. But Calebin-A showed higher ability in inhibiting the growth of HepG2 cells than Curcumin at the same concentration. After 48 h treatment, the IC50 of Curcumin and Calebin-A on HepG2 cells were about 25, and 10 μmol/L respectively. When the cells were exposed to 25 μmol/L Curcumin and Calebin-A, Calebin-A showed higher ability to inhibit the colony formation of HepG2 cells than Curcumin. Calebin-A induced the apoptosis of more HepG2 cells compared with Curcumin at the same concentration. CONCLUSION: Both Curcumin and Calebin-A could inhibit proliferation of HepG2 cells. But the new Curcuma longa constituent Calebin-A shows higher ability in inhibiting the growth of HepG2 cells than Curcumin.
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NSTL
万方数据
维普
